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Table 3.
Genes whose deletion confers hypersensitivity to caspofungin
|
|
|
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|
Caspofungin sensitivitya
|
| ORF |
Gene |
Comment |
Haploid |
Heterozygous diploid |
|
| Cell wall components synthesis and cell wall assembly |
| YBR023C |
CHS3 |
Chitin synthase III |
- - |
- - |
| YBL061C |
CHS4 |
Activator of Chs3p |
- - |
|
| YLR330W |
CHS5 |
Involved in Chs3p transport from the late Golgi to the chitosome |
- - |
|
| YJL099W |
CHS6 |
Involved in Chs3p transport from the late Golgi to the chitosome |
- - |
|
| YHR142W |
CHS7 |
Facilitates exit of Chs3p from the ER |
- - |
|
| YLR342W |
FKS1 |
ß-1,3-Glucan synthase subunit |
- - - |
- - - |
| YDR245W |
MNN10 |
Subunit of the Mannan polymerase II complex |
- |
|
| YDL047W |
SIT4 |
Protein phosphatase that negatively regulates Slt2p |
- |
|
| YGR229C |
SMI1 |
Regulator of ß-1,3-glucan synthesis |
- |
- - - |
| Cytoskeleton assembly and vesicular transport |
| YIL142W |
CCT2 |
Subunit of the TCP-1 ring complex required for the folding of actin and tubulin |
NA |
- - |
| YJR064W |
CCT5 |
Subunit of the TCP-1 ring complex required for the folding of actin and tubulin |
NA |
- - - |
| YJL204C |
RCY1 |
Involved in endocytic membrane traffic and recycling out of an early endosome |
- - |
|
| YBL007C |
SLA1 |
Cortical actin patch assembly control protein, mutation affects endocytosis |
- |
0 |
| YPR139C |
VPS66 |
Class B vacuolar sorting protein |
- |
|
| Transcription and protein synthesis |
| YER017C |
AFG3 |
Mitochondrial protease, degrades nonassembled inner membrane proteins |
- |
- - |
| YHR013C |
ARD1 |
Protein N-acetyltransferase |
- |
|
| YDR173C |
ARG82 |
Inositol phosphate kinase |
- - |
|
| YDR364C |
CDC40 |
Protein required for the second catalytic step of mRNA splicing |
- - |
|
| YDR432W |
NPL3 |
Involved in nuclear export of poly(A)+ mRNA |
- - |
- - - |
| YIL021W |
RPB3 |
RNA polymerase II subunit |
NA |
- - |
| YBL014C |
RRN6 |
RNA polymerase I core transcription factor |
NA |
- - - |
| YGR215W |
RSM27 |
Component of mitochondrial ribosomal subunit |
- - |
|
| YOR290C |
SNF2 |
Transcription factor acting in the SWI/SNF chromatin remodeling complex |
- |
0 |
| YJL127C |
SPT10 |
General transcription repressor |
- |
|
| YLR182W |
SWI6 |
Component of SBF and MBF transcription factors, G1/S transition |
- |
0 |
| YOL072W |
THP1 |
Transcription factor involved in transcription elongation |
- - - |
- - - |
| YGR285C |
ZUO1 |
Component of ribosome-associated complex |
- - |
- - - |
| YHR085W |
|
May be involved in rRNA processing |
NA |
- - |
| Other and unknown functions |
| YEL027W |
CUP5 |
Component of V0 sector of the vacuolar H+-ATPase. |
- - |
- - - |
| YDR052C |
DBF4 |
Regulatory subunit of Cdc7p kinase, required for G1/S transition |
NA |
- - - |
| YEL046C |
GLY1 |
Threonine aldolase, required for glycine biosynthesis |
- |
- - |
| YJR118C |
ILM1 |
Null mutant shows increased loss of mitochondrial DNA |
- - |
- - - |
| YNL126W |
SPC98 |
Spindle pole body component that interacts with -tubulin |
NA |
- - |
| YGR105W |
VMA21 |
Required for export of V-ATPase V0 sector out of the ER |
- - |
|
a Scores for caspofungin sensitivity are as follows: - - -, no growth of haploid on 100 ng/ml, no growth of heterozygous diploid on 150 ng/ml; - -, slow growth of haploid on 100 ng/ml and no growth on 200 ng/ml, slow growth of heterozygous diploid on 150 ng/ml; -, slow growth of haploid on 200 ng/ml; 0, wild-type growth; NA, nonapplicable (essential gene).
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