Table 2. Genes showing synthetic interaction with FKS1, FKS2, GAS1, or SMI1

ORF Gene Description of gene product Interaction

Cell wall synthesis and regulation (31 genes)
YJL095W BCK1 MAPKK-kinase of the cell integrity pathway GAS1, SMI1
YER155C BEM2 GTPase-activating protein for Rho1p FKS1, GAS1, SMI1
YNL233W BNI4 Anchors Chs3p-Chs4p to the bud neck septin ring FKS1, SMI1
YBR023C CHS3 Chitin synthase III FKS1, GAS1, SMI1
YBL061C CHS4 Activator of Chs3p FKS1, SMI1
YLR330W CHS5 Involved in transport of Chs3p from the late Golgi to the chitosome FKS1, GAS1, SMI1
YJL099W CHS6 Involved in transport of Chs3p from the late Golgi to the chitosome FKS1, SMI1
YHR142W CHS7 Facilitates exit of Chs3p from the ER FKS1, SMI1
YGL027C CWH41 Glucosidase I, mutant has a reduced level of ß-1,6-glucan GAS1
YOR030W DFG16 Involved in invasive growth, mutant hypersensitive to calcofluor white FKS1, SMI1
YBL101C ECM21 Mutant hypersensitive to calcofluor white SMI1
YLR443W ECM7 Mutant hypersensitive to calcofluor white GAS1
YLR342W FKS1 ß-1,3-Glucan synthase subunit FKS2
YGR032W FKS2 ß-1,3-Glucan synthase subunit FKS1
YLL043W FPS1 Glycerol channel protein FKS1, SMI1
YMR307W GAS1 GPI-anchored surface glycoprotein with ß-1,3-glucanosyltransferase activity SMI1
YCR046C IMG1 Putative mitochondrial ribosomal protein, null mutant K1 toxin resistant GAS1
YNL322C KRE1 Cell wall protein involved in ß-1,6-glucan synthesis GAS1, SMI1
YLR332W MID2 Sensor for the PKC1-SLT2 cell wall integrity pathway FKS1, SMI1
YDR245W MNN10 Subunit of the Mannan polymerase II complex SMI1
YJL183W MNN11 Subunit of the Mannan polymerase II complex FKS1, SMI1
YOR275C RIM20 Transcription factor involved in stress resistance FKS1, SMI1
YPL089C RLM1 Transcription factor mediating cell integrity pathway response FKS1, GAS1, SMI1
YLR371W ROM2 GDP/GTP exchange factor for Rho1p FKS1, GAS1, SMI1
YBR229C ROT2 Glucosidase II, mutant has a reduced level of ß-1,6-glucan GAS1
YOR008C SLG1 Sensor for the PKC1-SLT2 cell integrity pathway GAS1
YHR030C SLT2 MAP-kinase of the cell integrity pathway FKS1, GAS1, SMI1
YGR229C SMI1 Regulator of ß-1,3-glucan synthesis GAS1
YDR293C SSD1 mRNA-binding protein, may regulate expression of cell integrity pathway targets GAS1
YER111C SWI4 Transcription factor mediating the cell integrity pathway response FKS1, GAS1, SMI1
YML115C VAN1 Component of the Mannan polymerase I complex SMI1
Polarity and secretory pathway function (29 genes)
YBR200W BEM1 SH3-domain protein maintaining Cdc42p-Cdc24p at the bud tip SMI1
YPL161C BEM4 Bud emergence protein that activates Cdc42p FKS1, GAS1
YNL271C BNI1 Member of the polarisome with Bud6p, Pea2p, and Spa2p. This complex binds activated Cdc42p and its effector Ste20p and acts as an apical scaffold regulating actin filament assembly at the bud tip FKS1, SMI1
YNR051C BRE5 Activator of Ubp3p that regulates COPII coat assembly FKS1, SMI1
YJL188C BUD19 Mutant defective in bud site selection and bipolar budding GAS1, SMI1
YBR131W CCZ1 Protein involved in vesicular transport and vacuolar assembly SMI1
YNL298W CLA4 Cdc42p effector regulating septin assembly at the bud neck SMI1
YPR030W CSR2 Overexpression rescues chs5 spa2 synthetic lethality FKS1
YBL047C EDE1 Cortical actin patch protein with a role in endocytosis FKS1
YKL048C ELM1 Serine/threonine protein kinase regulating septin network organization FKS1, SMI1
YGL200C EMP24 COPII-coated vesicle protein required for sorting of GPI-anchored proteins GAS1
YBR041W FAT1 Very long-chain acyl-CoA synthetase SMI1
YCR034W FEN1 Involved in fatty acids elongation FKS1
YDL223C HBT1 Target of Hub1p ubiquitination, mutant shows morphological defects FKS1
YGR166W KRE11 Subunit of transport protein particle II complex GAS1, SMI1
YJR073C OPI3 Involved in phosphatidylcholine biosynthesis GAS1
YDR137W RGP1 Acts in a complex with Ric1p as a GTP-GDP exchange factor for Ypt6p FKS1, GAS1
YLR039C RIC1 Acts in a complex with Rgp1p as a GTP-GDP exchange factor for Ypt6p GAS1, SMI1
YDR388W RVS167 Affects cortical actin patch distribution, mutant shows defective endocytosis FKS1, SMI1
YDR351W SBE2 Golgi protein involved in targeting Chs3p and Chr2p to the bud neck SMI1
YOR035C SHE4 Involved in cortical actin patch assembly and endocytosis FKS1
YBL007C SLA1 Cortical actin patch assembly control protein, mutation affects endocytosis FKS1, SMI1
YLL021W SPA2 Member of the polarisome with Bni1p, Bud6p, and Pea2. This complex binds activated Cdc42p and its effector Ste20p and acts as an apical scaffold regulating actin filament assembly at the bud tip FKS1, SMI1
YER151C UBP3 Ubiquitin-specific protease, Ubp3p-Bre5p regulates COPII coat assembly FKS1, SMI1
YDR136C VPS61 Class B vacuolar sorting protein GAS1
YLR261C VPS63 Vacuolar protein sorting GAS1
YKR020W VPS67 Involved in maintenance of actin cytoskeleton and apical bud growth FKS1, SMI1
YLR262C YPT6 GTP-binding protein that regulates vesicle fusion during retrograde transport GAS1, SMI1
YLR338W Questionable ORF, overlaps with VRP1, a cortical actin patch component FKS1
Transcriptional regulation and stress response (22 genes)
YDL243C AAD4 Putative aryl-alcohol dehydrogenase GAS1
YNL027W CRZ1 Calcineurin responsive zinc-finger transcription factor FKS1
YKR024C DBP7 RNA helicase required for 60S ribosomal subunit assembly FKS1
YBR121C GRS1 Glycyl-tRNA synthetase, also involved in 3'-end formation of mRNA FKS1
YOR358W HAP5 Transcription factor required for activity of the CCAAT-binding complex GAS1
YJR055W HIT1 Required for growth at high temperature FKS1
YLR384C IKI3 RNA polymerase II transcriptional elongation component SMI1
YIL154C IMP2' Transcriptional activator with a role in DNA repair upon oxidative stress FKS1
YKL032C IXR1 Transcription factor mediating oxygen repression FKS1
YJL124C LSM1 Sm-like RNA-binding protein involved in control of mRNA decay FKS1
YDR378C LSM6 U6 snRNA-associated protein of the Sm-like group FKS1
YMR038C LYS7 Copper chaperone essential for the oxidative protective function of Sod1p SMI1
YML017W PSP2 Suppressor of DNA polymerase {alpha} mutation FKS1
YLR204W QRI5 Transcription profile suggests involvement in stress responses FKS1
YDR156W RPA14 RNA polymerase I subunit A14 SMI1
YLR357W RSC2 Component of the RSC chromatin remodeling complex regulating RNA polymerase II and III transcription GAS1
YKR072C SIS2 Involved in ion homeostasis GAS1
YDR477W SNF1 Protein kinase with roles in glucose derepression and filamentation FKS1
YOL006C TOP1 DNA topoisomerase I FKS1
YNL064C YDJ1 Hsp40 chaperone required for protein entry into the ER FKS1
YJL046W Lipoate-protein ligase A-related, null mutant hypersensitive to oxidative stress FKS1
YMR073C Possible NAD(P)H oxidoreductase involved in stress response FKS1
Ion homeostasis and signal transduction (5 genes)
YGR217W CCH1 Calcium channel protein FKS1, SMI1
YKL190W CNB1 Regulatory subunit of the phosphatase calcineurin FKS1, GAS1, SMI1
YJL117W PHO86 ER protein facilitating incorporation of Pho84p into secretory vesicles SMI1
YDL006W PTC1 Protein phosphatase, acts on MAP kinases such as Hog1p FKS1, GAS1, SMI1
YEL031W SPF1 ATP-dependent calcium pump required for normal ER calcium homeostasis SMI1
Ubiquitin-regulated protein degradation (4 genes)
YGR133W PEX4 E2 ubiquitin-conjugating enzyme induced in the unfolded protein response FKS1
YGR135W PRE9 20S (catalytic) proteasome subunit Y13 ({alpha}3) FKS1, SMI1
YHR200W RPN10 19S (regulatory) proteasome subunit, homolog of the mammalian S5a protein FKS1, SMI1
YBR082C UBC4 E2 ubiquitin-conjugating enzyme FKS1, SMI1
Cell cycle (3 genes)
YGL240W DOC1 Component of the anaphase-promoting complex FKS1, SMI1
YPL031C PHO85 Cyclin-dependent protein kinase that interacts with cyclin Pho80p FKS1, GAS1, SMI1
YNL171C Overlaps with APC1, an essential subunit of the anaphase-promoting complex FKS1, SMI1
Other (17 genes)
YHR129C ARP1 Centractin SMI1
YMR116C ASC1 40S ribosomal protein that affects cell size SMI1
YPR135W CTF4 DNA-directed DNA polymerase {alpha}-binding protein SMI1
YHR191C CTF8 Putative kinetochore protein SMI1
YDR440W DOT1 Involved in telomere silencing and in the meiotic arrest checkpoint GAS1
YDR508C GNP1 High-affinity glutamine permease FKS1, GAS1
YDR162C NBP2 Nap1p-binding protein GAS1, SMI1
YHR004C NEM1 Protein required for nuclear morphology GAS1
YJL208C NUC1 Nuclease, mitochondrial FKS1
YER178W PDA1 Pyruvate dehydrogenase complex E1-{alpha}-subunit SMI1
YMR205C PFK2 ß-subunit of the 6-phosphofructokinase SMI1
YNL069C RPL16B Ribosomal protein L16, nearly identical to Rpl16Ap FKS1
YOR312C RPL20B Ribosomal protein L20, nearly identical to Rpl20Ap FKS1
YFR032C-A RPL29 Ribosomal protein L29 SMI1
YGR214W RPS0A 40S ribosomal protein SMI1
YJL136C RPS21B Ribosomal protein S21B FKS1
YBR166C TYR1 Prephenate dehydrogenase in the tyrosine biosynthesis pathway SMI1
Unknown (24 genes)
YMR318C ADH6 NADPH-dependent cinnamyl-alcohol dehydrogenase GAS1
YJR118C ILM1 Null mutant shows increased loss of mitochondrial DNA FKS1, SMI1
YLR320W MMS22 Unknown FKS1, SMI1
YNL294C RIM21 Protein of unknown function FKS1, GAS1
YAL053W Expression is upregulated in an fks1 mutant GAS1
YBL062W Questionable ORF, overlaps with CHS4 FKS1
YGL046W Hypothetical protein, merged with RIM8/YGL045W FKS1, SMI1
YGL081W Phosphopeptide-binding protein with a forkhead-associated (FHA) domain FKS1
YGL110C Member of the CUE domain family, which binds ubiquitin-conjugating enzymes FKS1, GAS1
YGL196W Hypothetical protein FKS1
YGR237C Weak similarity to YOR019W SMI1
YIL121W Member of the multidrug-resistance 12-spanner family GAS1
YLR021W Hypothetical protein FKS1
YLR358C Unknown SMI1
YML117W Unknown GAS1, SMI1
YMR313C Unknown GAS1
YMR316C-A Protein of unknown function GAS1
YMR317W Unknown GAS1
YMR326C Protein with high similarity to Ynr077p GAS1
YOL003C Similar to Erf2p, Ydr459p, and others in a cysteine-rich domain FKS1, SMI1
YPL041C Hypothetical protein FKS1, GAS1
YPL077C Weak similarity to YBR197C SMI1
YPL144W Unknown FKS1
YPL261C Putative GPI-anchored protein that may be involved in cell wall maintenance FKS1
ORF, open reading frame; ER, endoplasmic reticulum.