|
Table 2.
Genes showing synthetic interaction with FKS1, FKS2, GAS1, or SMI1
|
|
| ORF |
Gene |
Description of gene product |
Interaction |
|
| Cell wall synthesis and regulation (31 genes) |
| YJL095W |
BCK1 |
MAPKK-kinase of the cell integrity pathway |
GAS1, SMI1 |
| YER155C |
BEM2 |
GTPase-activating protein for Rho1p |
FKS1, GAS1, SMI1 |
| YNL233W |
BNI4 |
Anchors Chs3p-Chs4p to the bud neck septin ring |
FKS1, SMI1 |
| YBR023C |
CHS3 |
Chitin synthase III |
FKS1, GAS1, SMI1 |
| YBL061C |
CHS4 |
Activator of Chs3p |
FKS1, SMI1 |
| YLR330W |
CHS5 |
Involved in transport of Chs3p from the late Golgi to the chitosome |
FKS1, GAS1, SMI1 |
| YJL099W |
CHS6 |
Involved in transport of Chs3p from the late Golgi to the chitosome |
FKS1, SMI1 |
| YHR142W |
CHS7 |
Facilitates exit of Chs3p from the ER |
FKS1, SMI1 |
| YGL027C |
CWH41 |
Glucosidase I, mutant has a reduced level of ß-1,6-glucan |
GAS1 |
| YOR030W |
DFG16 |
Involved in invasive growth, mutant hypersensitive to calcofluor white |
FKS1, SMI1 |
| YBL101C |
ECM21 |
Mutant hypersensitive to calcofluor white |
SMI1 |
| YLR443W |
ECM7 |
Mutant hypersensitive to calcofluor white |
GAS1 |
| YLR342W |
FKS1 |
ß-1,3-Glucan synthase subunit |
FKS2 |
| YGR032W |
FKS2 |
ß-1,3-Glucan synthase subunit |
FKS1 |
| YLL043W |
FPS1 |
Glycerol channel protein |
FKS1, SMI1 |
| YMR307W |
GAS1 |
GPI-anchored surface glycoprotein with ß-1,3-glucanosyltransferase activity |
SMI1 |
| YCR046C |
IMG1 |
Putative mitochondrial ribosomal protein, null mutant K1 toxin resistant |
GAS1 |
| YNL322C |
KRE1 |
Cell wall protein involved in ß-1,6-glucan synthesis |
GAS1, SMI1 |
| YLR332W |
MID2 |
Sensor for the PKC1-SLT2 cell wall integrity pathway |
FKS1, SMI1 |
| YDR245W |
MNN10 |
Subunit of the Mannan polymerase II complex |
SMI1 |
| YJL183W |
MNN11 |
Subunit of the Mannan polymerase II complex |
FKS1, SMI1 |
| YOR275C |
RIM20 |
Transcription factor involved in stress resistance |
FKS1, SMI1 |
| YPL089C |
RLM1 |
Transcription factor mediating cell integrity pathway response |
FKS1, GAS1, SMI1 |
| YLR371W |
ROM2 |
GDP/GTP exchange factor for Rho1p |
FKS1, GAS1, SMI1 |
| YBR229C |
ROT2 |
Glucosidase II, mutant has a reduced level of ß-1,6-glucan |
GAS1 |
| YOR008C |
SLG1 |
Sensor for the PKC1-SLT2 cell integrity pathway |
GAS1 |
| YHR030C |
SLT2 |
MAP-kinase of the cell integrity pathway |
FKS1, GAS1, SMI1 |
| YGR229C |
SMI1 |
Regulator of ß-1,3-glucan synthesis |
GAS1 |
| YDR293C |
SSD1 |
mRNA-binding protein, may regulate expression of cell integrity pathway targets |
GAS1 |
| YER111C |
SWI4 |
Transcription factor mediating the cell integrity pathway response |
FKS1, GAS1, SMI1 |
| YML115C |
VAN1 |
Component of the Mannan polymerase I complex |
SMI1 |
| Polarity and secretory pathway function (29 genes) |
| YBR200W |
BEM1 |
SH3-domain protein maintaining Cdc42p-Cdc24p at the bud tip |
SMI1 |
| YPL161C |
BEM4 |
Bud emergence protein that activates Cdc42p |
FKS1, GAS1 |
| YNL271C |
BNI1 |
Member of the polarisome with Bud6p, Pea2p, and Spa2p. This complex binds activated Cdc42p and its effector Ste20p and acts as an apical scaffold regulating actin filament assembly at the bud tip |
FKS1, SMI1 |
| YNR051C |
BRE5 |
Activator of Ubp3p that regulates COPII coat assembly |
FKS1, SMI1 |
| YJL188C |
BUD19 |
Mutant defective in bud site selection and bipolar budding |
GAS1, SMI1 |
| YBR131W |
CCZ1 |
Protein involved in vesicular transport and vacuolar assembly |
SMI1 |
| YNL298W |
CLA4 |
Cdc42p effector regulating septin assembly at the bud neck |
SMI1 |
| YPR030W |
CSR2 |
Overexpression rescues chs5 spa2 synthetic lethality |
FKS1 |
| YBL047C |
EDE1 |
Cortical actin patch protein with a role in endocytosis |
FKS1 |
| YKL048C |
ELM1 |
Serine/threonine protein kinase regulating septin network organization |
FKS1, SMI1 |
| YGL200C |
EMP24 |
COPII-coated vesicle protein required for sorting of GPI-anchored proteins |
GAS1 |
| YBR041W |
FAT1 |
Very long-chain acyl-CoA synthetase |
SMI1 |
| YCR034W |
FEN1 |
Involved in fatty acids elongation |
FKS1 |
| YDL223C |
HBT1 |
Target of Hub1p ubiquitination, mutant shows morphological defects |
FKS1 |
| YGR166W |
KRE11 |
Subunit of transport protein particle II complex |
GAS1, SMI1 |
| YJR073C |
OPI3 |
Involved in phosphatidylcholine biosynthesis |
GAS1 |
| YDR137W |
RGP1 |
Acts in a complex with Ric1p as a GTP-GDP exchange factor for Ypt6p |
FKS1, GAS1 |
| YLR039C |
RIC1 |
Acts in a complex with Rgp1p as a GTP-GDP exchange factor for Ypt6p |
GAS1, SMI1 |
| YDR388W |
RVS167 |
Affects cortical actin patch distribution, mutant shows defective endocytosis |
FKS1, SMI1 |
| YDR351W |
SBE2 |
Golgi protein involved in targeting Chs3p and Chr2p to the bud neck |
SMI1 |
| YOR035C |
SHE4 |
Involved in cortical actin patch assembly and endocytosis |
FKS1 |
| YBL007C |
SLA1 |
Cortical actin patch assembly control protein, mutation affects endocytosis |
FKS1, SMI1 |
| YLL021W |
SPA2 |
Member of the polarisome with Bni1p, Bud6p, and Pea2. This complex binds activated Cdc42p and its effector Ste20p and acts as an apical scaffold regulating actin filament assembly at the bud tip |
FKS1, SMI1 |
| YER151C |
UBP3 |
Ubiquitin-specific protease, Ubp3p-Bre5p regulates COPII coat assembly |
FKS1, SMI1 |
| YDR136C |
VPS61 |
Class B vacuolar sorting protein |
GAS1 |
| YLR261C |
VPS63 |
Vacuolar protein sorting |
GAS1 |
| YKR020W |
VPS67 |
Involved in maintenance of actin cytoskeleton and apical bud growth |
FKS1, SMI1 |
| YLR262C |
YPT6 |
GTP-binding protein that regulates vesicle fusion during retrograde transport |
GAS1, SMI1 |
| YLR338W |
|
Questionable ORF, overlaps with VRP1, a cortical actin patch component |
FKS1 |
| Transcriptional regulation and stress response (22 genes) |
| YDL243C |
AAD4 |
Putative aryl-alcohol dehydrogenase |
GAS1 |
| YNL027W |
CRZ1 |
Calcineurin responsive zinc-finger transcription factor |
FKS1 |
| YKR024C |
DBP7 |
RNA helicase required for 60S ribosomal subunit assembly |
FKS1 |
| YBR121C |
GRS1 |
Glycyl-tRNA synthetase, also involved in 3'-end formation of mRNA |
FKS1 |
| YOR358W |
HAP5 |
Transcription factor required for activity of the CCAAT-binding complex |
GAS1 |
| YJR055W |
HIT1 |
Required for growth at high temperature |
FKS1 |
| YLR384C |
IKI3 |
RNA polymerase II transcriptional elongation component |
SMI1 |
| YIL154C |
IMP2' |
Transcriptional activator with a role in DNA repair upon oxidative stress |
FKS1 |
| YKL032C |
IXR1 |
Transcription factor mediating oxygen repression |
FKS1 |
| YJL124C |
LSM1 |
Sm-like RNA-binding protein involved in control of mRNA decay |
FKS1 |
| YDR378C |
LSM6 |
U6 snRNA-associated protein of the Sm-like group |
FKS1 |
| YMR038C |
LYS7 |
Copper chaperone essential for the oxidative protective function of Sod1p |
SMI1 |
| YML017W |
PSP2 |
Suppressor of DNA polymerase mutation |
FKS1 |
| YLR204W |
QRI5 |
Transcription profile suggests involvement in stress responses |
FKS1 |
| YDR156W |
RPA14 |
RNA polymerase I subunit A14 |
SMI1 |
| YLR357W |
RSC2 |
Component of the RSC chromatin remodeling complex regulating RNA polymerase II and III transcription |
GAS1 |
| YKR072C |
SIS2 |
Involved in ion homeostasis |
GAS1 |
| YDR477W |
SNF1 |
Protein kinase with roles in glucose derepression and filamentation |
FKS1 |
| YOL006C |
TOP1 |
DNA topoisomerase I |
FKS1 |
| YNL064C |
YDJ1 |
Hsp40 chaperone required for protein entry into the ER |
FKS1 |
| YJL046W |
|
Lipoate-protein ligase A-related, null mutant hypersensitive to oxidative stress |
FKS1 |
| YMR073C |
|
Possible NAD(P)H oxidoreductase involved in stress response |
FKS1 |
| Ion homeostasis and signal transduction (5 genes) |
| YGR217W |
CCH1 |
Calcium channel protein |
FKS1, SMI1 |
| YKL190W |
CNB1 |
Regulatory subunit of the phosphatase calcineurin |
FKS1, GAS1, SMI1 |
| YJL117W |
PHO86 |
ER protein facilitating incorporation of Pho84p into secretory vesicles |
SMI1 |
| YDL006W |
PTC1 |
Protein phosphatase, acts on MAP kinases such as Hog1p |
FKS1, GAS1, SMI1 |
| YEL031W |
SPF1 |
ATP-dependent calcium pump required for normal ER calcium homeostasis |
SMI1 |
| Ubiquitin-regulated protein degradation (4 genes) |
| YGR133W |
PEX4 |
E2 ubiquitin-conjugating enzyme induced in the unfolded protein response |
FKS1 |
| YGR135W |
PRE9 |
20S (catalytic) proteasome subunit Y13 ( 3) |
FKS1, SMI1 |
| YHR200W |
RPN10 |
19S (regulatory) proteasome subunit, homolog of the mammalian S5a protein |
FKS1, SMI1 |
| YBR082C |
UBC4 |
E2 ubiquitin-conjugating enzyme |
FKS1, SMI1 |
| Cell cycle (3 genes) |
| YGL240W |
DOC1 |
Component of the anaphase-promoting complex |
FKS1, SMI1 |
| YPL031C |
PHO85 |
Cyclin-dependent protein kinase that interacts with cyclin Pho80p |
FKS1, GAS1, SMI1 |
| YNL171C |
|
Overlaps with APC1, an essential subunit of the anaphase-promoting complex |
FKS1, SMI1 |
| Other (17 genes) |
| YHR129C |
ARP1 |
Centractin |
SMI1 |
| YMR116C |
ASC1 |
40S ribosomal protein that affects cell size |
SMI1 |
| YPR135W |
CTF4 |
DNA-directed DNA polymerase -binding protein |
SMI1 |
| YHR191C |
CTF8 |
Putative kinetochore protein |
SMI1 |
| YDR440W |
DOT1 |
Involved in telomere silencing and in the meiotic arrest checkpoint |
GAS1 |
| YDR508C |
GNP1 |
High-affinity glutamine permease |
FKS1, GAS1 |
| YDR162C |
NBP2 |
Nap1p-binding protein |
GAS1, SMI1 |
| YHR004C |
NEM1 |
Protein required for nuclear morphology |
GAS1 |
| YJL208C |
NUC1 |
Nuclease, mitochondrial |
FKS1 |
| YER178W |
PDA1 |
Pyruvate dehydrogenase complex E1- -subunit |
SMI1 |
| YMR205C |
PFK2 |
ß-subunit of the 6-phosphofructokinase |
SMI1 |
| YNL069C |
RPL16B |
Ribosomal protein L16, nearly identical to Rpl16Ap |
FKS1 |
| YOR312C |
RPL20B |
Ribosomal protein L20, nearly identical to Rpl20Ap |
FKS1 |
| YFR032C-A |
RPL29 |
Ribosomal protein L29 |
SMI1 |
| YGR214W |
RPS0A |
40S ribosomal protein |
SMI1 |
| YJL136C |
RPS21B |
Ribosomal protein S21B |
FKS1 |
| YBR166C |
TYR1 |
Prephenate dehydrogenase in the tyrosine biosynthesis pathway |
SMI1 |
| Unknown (24 genes) |
| YMR318C |
ADH6 |
NADPH-dependent cinnamyl-alcohol dehydrogenase |
GAS1 |
| YJR118C |
ILM1 |
Null mutant shows increased loss of mitochondrial DNA |
FKS1, SMI1 |
| YLR320W |
MMS22 |
Unknown |
FKS1, SMI1 |
| YNL294C |
RIM21 |
Protein of unknown function |
FKS1, GAS1 |
| YAL053W |
|
Expression is upregulated in an fks1 mutant |
GAS1 |
| YBL062W |
|
Questionable ORF, overlaps with CHS4 |
FKS1 |
| YGL046W |
|
Hypothetical protein, merged with RIM8/YGL045W |
FKS1, SMI1 |
| YGL081W |
|
Phosphopeptide-binding protein with a forkhead-associated (FHA) domain |
FKS1 |
| YGL110C |
|
Member of the CUE domain family, which binds ubiquitin-conjugating enzymes |
FKS1, GAS1 |
| YGL196W |
|
Hypothetical protein |
FKS1 |
| YGR237C |
|
Weak similarity to YOR019W |
SMI1 |
| YIL121W |
|
Member of the multidrug-resistance 12-spanner family |
GAS1 |
| YLR021W |
|
Hypothetical protein |
FKS1 |
| YLR358C |
|
Unknown |
SMI1 |
| YML117W |
|
Unknown |
GAS1, SMI1 |
| YMR313C |
|
Unknown |
GAS1 |
| YMR316C-A |
|
Protein of unknown function |
GAS1 |
| YMR317W |
|
Unknown |
GAS1 |
| YMR326C |
|
Protein with high similarity to Ynr077p |
GAS1 |
| YOL003C |
|
Similar to Erf2p, Ydr459p, and others in a cysteine-rich domain |
FKS1, SMI1 |
| YPL041C |
|
Hypothetical protein |
FKS1, GAS1 |
| YPL077C |
|
Weak similarity to YBR197C |
SMI1 |
| YPL144W |
|
Unknown |
FKS1 |
| YPL261C |
|
Putative GPI-anchored protein that may be involved in cell wall maintenance |
FKS1 |
|
ORF, open reading frame; ER, endoplasmic reticulum.
|