Table 3. Genes whose deletion confers hypersensitivity to caspofungin

Caspofungin sensitivitya

ORF Gene Comment Haploid Heterozygous diploid

Cell wall components synthesis and cell wall assembly
YBR023C CHS3 Chitin synthase III - - - -
YBL061C CHS4 Activator of Chs3p - -
YLR330W CHS5 Involved in Chs3p transport from the late Golgi to the chitosome - -
YJL099W CHS6 Involved in Chs3p transport from the late Golgi to the chitosome - -
YHR142W CHS7 Facilitates exit of Chs3p from the ER - -
YLR342W FKS1 ß-1,3-Glucan synthase subunit - - - - - -
YDR245W MNN10 Subunit of the Mannan polymerase II complex -
YDL047W SIT4 Protein phosphatase that negatively regulates Slt2p -
YGR229C SMI1 Regulator of ß-1,3-glucan synthesis - - - -
Cytoskeleton assembly and vesicular transport
YIL142W CCT2 Subunit of the TCP-1 ring complex required for the folding of actin and tubulin NA - -
YJR064W CCT5 Subunit of the TCP-1 ring complex required for the folding of actin and tubulin NA - - -
YJL204C RCY1 Involved in endocytic membrane traffic and recycling out of an early endosome - -
YBL007C SLA1 Cortical actin patch assembly control protein, mutation affects endocytosis - 0
YPR139C VPS66 Class B vacuolar sorting protein -
Transcription and protein synthesis
YER017C AFG3 Mitochondrial protease, degrades nonassembled inner membrane proteins - - -
YHR013C ARD1 Protein N-acetyltransferase -
YDR173C ARG82 Inositol phosphate kinase - -
YDR364C CDC40 Protein required for the second catalytic step of mRNA splicing - -
YDR432W NPL3 Involved in nuclear export of poly(A)+ mRNA - - - - -
YIL021W RPB3 RNA polymerase II subunit NA - -
YBL014C RRN6 RNA polymerase I core transcription factor NA - - -
YGR215W RSM27 Component of mitochondrial ribosomal subunit - -
YOR290C SNF2 Transcription factor acting in the SWI/SNF chromatin remodeling complex - 0
YJL127C SPT10 General transcription repressor -
YLR182W SWI6 Component of SBF and MBF transcription factors, G1/S transition - 0
YOL072W THP1 Transcription factor involved in transcription elongation - - - - - -
YGR285C ZUO1 Component of ribosome-associated complex - - - - -
YHR085W May be involved in rRNA processing NA - -
Other and unknown functions
YEL027W CUP5 Component of V0 sector of the vacuolar H+-ATPase. - - - - -
YDR052C DBF4 Regulatory subunit of Cdc7p kinase, required for G1/S transition NA - - -
YEL046C GLY1 Threonine aldolase, required for glycine biosynthesis - - -
YJR118C ILM1 Null mutant shows increased loss of mitochondrial DNA - - - - -
YNL126W SPC98 Spindle pole body component that interacts with {gamma}-tubulin NA - -
YGR105W VMA21 Required for export of V-ATPase V0 sector out of the ER - -
a Scores for caspofungin sensitivity are as follows: - - -, no growth of haploid on 100 ng/ml, no growth of heterozygous diploid on 150 ng/ml; - -, slow growth of haploid on 100 ng/ml and no growth on 200 ng/ml, slow growth of heterozygous diploid on 150 ng/ml; -, slow growth of haploid on 200 ng/ml; 0, wild-type growth; NA, nonapplicable (essential gene).